Small 11, 681-687 (2015).
Stripe‐Patterned Thermo‐responsive Cell Culture Dish for Cell Separation without Cell Labeling
Kumashiro Y, Ishihara J, Umemoto T, Itoga K, Kobayashi J, Shimizu T, Yamato M, Okano T
A stripe‐patterned thermo‐responsive surface is prepared to enable cell separation without labeling. The thermo‐responsive surface containing a 3 μm striped pattern exhibits various cell adhesion and detachment properties. A mixture of three cell types is separated on the patterned surface based on their distinct cell‐adhesion properties, and the composition of the cells is analyzed by flow cytometry.